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KMID : 0387420110220020163
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Korean Society of Oriental Neuropsychiatry
2011 Volume.22 No. 2 p.163 ~ p.176
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Effects of Water-Extracts of Bambusae concretio silicae(BCS, 天竺黃) on the Expression of Neurotransmitter Receptors
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Yoon Jae-Hong
Kim Kyung-Min
Kim Kyung-Hun
Shin Gil-Cho
Jeong Seung-Hyun
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Abstract
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Objectives : BCS(Bambusae concretio silicae) is used as a traditional medicine in Korea for the incipient stroke. Recent reports indicated that BCS has a neuroprotective effect by anti-convulsion effect. However, it’s mechanism is not well studied. The purpose of this study was to investigate into the molecular mechanism of BCS for neuroprotection in normoxia of cultured rat hippocampal neurons.
Methods : BCS (1.0, 2.5, 5.0, and ) was added to culture media (Neurobasal supplemented with B27) on DIV 0, given a normoxia, and the cell viability was measured by typical phase-contrast images of the cultures with 1.0, 2.5, 5.0, and on DIV 21. Effects of BCS on the expression of various synaptic proteins ( R1, R2, GlyR, PSD95) were observed by immunocytochemistry showing on DIV 3, 7 and 21.
Results : Typical phase-contrast images of the cultures indicated that BCS has a protective effect of rat hippocampal cells in normoxia. The BCS upregulated R1 after normoxia on DIV 7, on DIV 21 and R2 on DIV 21. And the BCS downregulated PSD95 after normoxia on DIV 7.
Conclusions : The present study showed evidence for the efficacy of BCS in Typical phase-contrast images, upregulation of inhibitory neurotransmitter receptors( R1) and downregulation of PSD95 which eventually protected neuronal cell death in normoxia.
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KEYWORD
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Bambusae concretio silicae, GABA[A] β2/3 , GABA[B] R1, GABA[B] R2, GlyR, PSD95, Normoxia
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